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Fig. 7. smp is involved in cell proliferation from MBT onwards.
Clonal injections with either control MOs (smpMOIC and
smpMOIIC) or specific MOs (smpMOI and smpMOII),
coupled with carboxyfluorescein (-F) or lissamine (-L), respectively.
(A-H) Injected embryos observed at early gastrula (A,D), mid gastrula
(B,E) or late gastrula (C,F) stages. (A,B) Animal and dorsal views of
control-injected embryos. (D,E) Animal and dorsal views of
smpMO-injected embryos, showing smp-morphant cells that
derived from the injected blastomere. Some of them remain aggregated during
gastrulation (white dotted lines indicate the margin of the blastoderm). (C,F)
At late gastrula stage, smpMO-containing cells are excluded from the
embryonic axis (white dotted lines delimit both sides of the body axes). (G)
Number of fluorescent cells per embryo, at early gastrula stage, within a
single experiment (n, number of embryos). The average number of
fluorescent cells is 67.3±4.3 for control embryos and 42.2±9.8
for smpMO-injected embryos. These values are significantly different
(P<0.02). (H) Half of the smpMOII-L-injected embryos
display a reduced number of isolated fluorescent cells (<50), always
associated with a single cluster of cells. (I-N) Confocal microscope
observation of flat mounts of early gastrula embryos after clonal injection
with smpMOIIC-L (I-K, n=3) or smpMOII-L (L-N,
n=5). The nuclear morphology of smpMO-containing cells (red)
was analyzed by DAPI staining (blue). Control-injected cells showed normal
mitotic (arrowhead) and interphasic (arrow and inset) nuclei (I-K). By
contrast, smpMOII-L-injected blastomeres have descendants with
abnormally large nuclei (L-N, arrows and inset).
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