spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 8


Fig. 8. smp regulates Ol-pou5f1 expression. (A,B) Transverse sections, after WMISH, showing Ol-pou5f1 expression in early gastrula embryos after smpMO injection (8 mg/ml). Decreased expression was observed in deep cells, but not in the EVL of injected embryos (arrowhead). (C-F) Embryos injected at the 32-cell stage with EGFP RNA (100 µg/ml), either alone (C,E) or together with smpRNA (500 µg/ml, D,F). At mid-gastrulation (C,D, dorsal view), no apparent modifications can be observed (white dotted lines indicate the blastoderm margin). At the 2-somite stage (E,F, dorsal view), fluorescent cells are excluded from the embryo axis and are found near the anterior body axis. (G) Lateral view of a 2-somite stage control embryo showing Ol-pou5f1 expression in telencephalon (t) and tailbud (tb). (H,I) Ol-pou5f1 expression at the 2-somite stage after smpRNA injection into two-cell stage embryos. (H) Ectopic expression is visible in the telencephalon (t), ventral mhb and rhombencephalic domains (rb). (I) A more widespread expression is observed in strongly affected embryos. (J-O) Tranverse sections of WMISH embryos. (J-L) Control embryos. Ol-pou5f1 CNS expression is restricted to the dorsal telencephalon (arrowhead). (M-O) After injection of smpRNA, ectopic Ol-pou5f1 expression was observed in telencephalon (M, arrowhead), and in the ventral mhb (N, arrowhead) and rhombencephalon (O). Scale bars: 30 µm.





Right arrow Return to article