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Figure 2


Fig. 2. Rescue of Raldh1-/-;Raldh3-/- eye morphogenetic movements by maternal dietary RA supplementation. (A-F) Raldh1-/- and Raldh1-/-;Raldh3-/- littermates were subjected to maternal dietary RA supplementation from E8.5 until the point of analysis, then analyzed by hematoxylin-eosin staining of frontal sections through the eye of E14.5 embryos (A,B), or RARE-lacZ expression in frontal sections of E11.5 (C,D) and E12.5 (E,F) eyes. (G-K) Whole-mount in situ hybridization to detect the expression of Raldh1, Raldh2 and Raldh3 in E10.5 whole-mount eyes (G-I), and Crabp1 and Crabp2 in E11.5 eyes following frontal sectioning (J,K). c, cornea; D, dorsal; el, eyelid; nr, neural retina; nr/vb, neural retina/vitreous body junction; olf, olfactory pit; pm, perioptic mesenchyme; rpe, retinal pigment epithelium; V, ventral; vb, vitreous body.





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