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Fig. 3. dgn-1 is expressed in epithelia and neurons. (A) In early embryos, dgn-1::GFP is expressed in epithelial and neural precursors in the outer layer of cells. (B) As elongation begins, expression becomes prominent in pharyngeal epithelia (pe), the excretory cell (exc), the two somatic gonad precursors in the gonad primordium (gp), and in rectal epithelia (rect). Weaker expression is seen in a number of hypodermal and neural precursors. (C) In early larvae, expression persists in the e2 and marginal (mc) cells of the pharyngeal epithelium, the excretory cell, the gonad primordium and the rectal epithelium. Strong expression is seen in the PVP neurons. (D-G) Enlarged images showing expression in: (D) the pharyngeal epithelium and hypodermal cell hyp5 (hyp); (E) somatic gonad precursors, which project processes (arrowheads) around the primordial germ cells (asterisks); (F) PVP neurons; (G) rectal epithelial cells, which project processes (arrowhead) around the rectal sphincter. (H) Transient expression during late L1 stage in P-cell-derived ventral cord neurons (arrowheads). (I) A L3 stage animal showing expression in the distal tip cell and gonad epithelium (arrowheads). (J) Strong expression in L4/adult stage gonad, including the uterus (ut), spermatheca (st) and sheath (sh). (K) Expression in late L4 stage vulval epithelium. Scale bar: 10 µm.

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