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Figure 6


Fig. 6. Localization of BM components to the dgn-1(0) gonad. (A,B) Wild-type (A) and cg121 homozygote (B) L1 larvae stained with antibodies to collagen {alpha}2(IV) LET-2 (green) and to DGN-1 (red). (C,D) Wild-type (C) and cg121 homozygote (D) L1 larvae stained with antibodies to nidogen NID-1 (green) and to DGN-1 (red). Early cg121 larvae retaining a compact gonad primordium show wild-type staining of the gonad primordium with LET-2 and NID-1 antibodies (arrows). (E) Early cg121 homozygote expressing LAM-1::GFP (green); association with the compact gonad primordium is apparent (enlarged with DIC overlay in inset). (F) Early cg121 homozygote with a mispositioned SGP; both SGPs with an associated strong LAM-1::GFP signal (green) ensheath only one (arrowheads) of the two primordial germ cells (asterisks). The unsheathed PGC is only weakly associated with laminin (arrow). (G) DIC image of F. Scale bar: 10 µm.





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