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Figure 2


Fig. 2. Reduced PGCs and phosphoSmad staining in Bmp4S2G/S2G embryos. (A) Graph of PGC number versus somite pairs for wild-type, Bmp4S2G/+ and Bmp4S2G/S2G embryos. (B) Immunostaining of E6 Bmp4+/+ and Bmp4S2G/S2G embryo sections with an antibody that recognizes the phosphorylated forms of SMAD1, 5 and 8. Staining in the proximal epiblast (arrows) and the visceral endoderm (arrowheads) is decreased in the Bmp4S2G/S2G embryo.





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