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Files in this Data Supplement:
Fig. S1. Capsulin expression in stage 15-17 chick embryos. (A-C) Whole-mount in situ hybridization for the bHLH transcription factor Capsulin. Lateral left views are shown in A and C; ventral view is shown in B. Note the expression of Capsulin in the distal region of the outflow tract (white arrows). ba1, branchial arch 1; ba2, branchial arch 2; oft; outflow tract.
Fig. S2. Gene expression analysis of stage 16-17 chick embryos. (A-E) Whole-mount in situ hybridization for the transcription factor Tbx5 and the signaling molecules MAP kinase phosphatase (Mkp3), Fgf10, Bmp2 and Bmp7. (E1-E4) Transverse sections of an embryo hybridized for Bmp7 at four levels (first branchial arch, outflow tract/second branchial arch, epithelial splanchnic mesoderm/third branchial arch and inflow tract). ba1, branchial arch 1; ba2, branchial arch 2; end, endoderm; nt, neural tube; oft; outflow tract; ov, otic vesicle; ph, pharynx; spm, splanchnic mesoderm.
Fig. S3. Cranial paraxial mesoderm cells migrate through branchial arches 1 and 2 into the outflow tract. (A) DiI injected at stage 10 into the right CPM (A) at the level of rhombomers 4-5. (B) Lateral view of the embryo at stage 17; (C) transverse section (indicated by dotted line) at stage 17, shown as an overlay of bright field and fluorescence images.
Fig. S4. Cranial paraxial mesoderm cells contribute to the outflow tract. (A) RCAS-GFP concentrated viruses injected at stage 10 into the right CPM (indicated by arrowhead). (B) Lateral view of the embryo depicted in A, after a 40-hour incubation, shown as an overlay of bright field and fluorescence images. (C) Heart dissected from GFP-infected embryos showing GFP expression in the outer curvature of the outflow tract. ba1, branchial arch 1; ba2, branchial arch 2; cc, conus cordis; rv, right ventricle; ta, truncus arteriosus.
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