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Fig. 6. Cellular and molecular analyses of cranial paraxial mesoderm en route to
the heart. (A) Diagram of a stage 16 embryo (ventral view),
following removal of the heart. Dotted lines represent the three section
levels displayed in the lower panels. (B) Diagram of quail-chick
chimera assay. (C-I) Transverse sections at the first branchial arch
(ba) level (upper dotted line in A). (C1-I1) Transverse sections at the
aortic sac level (middle line). (C2-I2) Transverse sections at the
outflow tract (OFT) level (lower line). (C-C2) Sections of CM-DiI-labeled
cells at the aforementioned levels. (D-D2) Transverse sections through the
quail-chick chimera, followed by immunostaining with the quail-specific
antibody (QCPN, x10 bright field and fluorescence images)
(D'-D2') x20 fluorescence images of the marked area shown in
the x10 images (D-D2). (E-E2) Immunostaining for Myosin heavy chain
using MF20 antibody. (F-I2) Sections through embryos subjected to whole-mount
in situ hybridization with the indicated markers. aaa1, aortic arch artery 1;
aaa2, aortic arch artery 2; as, aortic sac; ba1, ba2, first, second branchial
arches; end, endocardium; myo, myocardium; nt, neural tube; ov, otic vesicle;
ph, pharynx.
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