Supplemental Figure 1
-
Fig. S1. Temporal expression profile of
endoderm-enriched transcripts. (A) The temporal expression profile of the 301
endoderm-enriched sequences in egg, stage 11 gastrula (St11) and stage 18
neurula (St18) based on microarray analysis. Hierarchical gene clustering shows
low expression in blue and high expression in red. Based on an arbitrary
1.5-fold or greater expression level threshold, transcripts were classified
into three groups. (B) Transcripts
with 1.5-fold greater expression in egg than stage 11, indicating significant
maternal expression. (C)
Transcripts with less than 1.5-fold difference in expression levels between egg
and stage 11. These included maternal genes as well as some zygotic genes with
low absolute expression levels. (D)
Transcripts with 1.5-fold greater expression in stage 11 than egg, representing
zygotic transcripts with little if any maternal expression. (B-D) Transcripts with 1.5-fold greater expression in
stage 11 than stage 18 are shown in green, those with 1.5-fold greater expression
in stage 18 than stage 11 are shown in blue and those transcripts with less
than 1.5-fold difference between stages 11 and 18 are shown in red. The
representative genes Vg1, VegT, Gata6, Sox17a,
and Mixer are shown (black lines).
