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Figure 4


Fig. 4. Expression of known zygotic endoderm regulators in Nodal-, Mixer- and Sox17- embryos. The expression of the known endoderm regulators Xnr1, Xnr2, Xnr4, Mix1, Mix2, Mixer, Bix1, Bix2, Bix3, Bix4, Gata4, Gata5, Gata6, Sox17{alpha}, Sox17ß, Edd and Hnf1ß from (A) the microarray and (B) real-time RT-PCR validation was determined from Nodal- (Cerberus-S injected 1 ng/embryo), Mixer- (Mixer morpholino injected 40 ng/embryo) and Sox17- (Sox17{alpha}1+{alpha}2+ß morpholino injected 20 ng each/embryo) embryos at stage 11. Both the array data and RT-PCR is plotted on a log scale. Changes in expression less than 1.4-fold up or down from the control are within the horizontal yellow bar. From these and the validation of more than 40 other genes changes in 1.4-fold were considered robust and were validated in over 75% of the cases.





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