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Fig. 6. Novel modes of endoderm gene regulation. (A-O) Each of the
301 endoderm-enriched transcripts was placed in a category based on how it was
regulated in Nodal- (N), Mixer- (M) and Sox17- (S)
embryos, based on the criteria of 1.4-fold change in expression levels
relative to stage 11 control embryo. Genes downregulated more than 1.4-fold in
Nodal-, Mixer- or Sox17- embryos were classified as
positively regulated by Nodal proteins (+N), Mixer (+M) or Sox17 (+S),
respectively. Genes upregulated more than 1.4-fold in Nodal-,
Mixer- or Sox17- embryos were classified as negatively
regulated (-), i.e. normally repressed, by Nodal proteins (-N), Mixer (-M) or
Sox17 (-S), respectively. Genes with less than a 1.4-fold change in expression
levels relative to controls were considered to be `not obviously regulated' by
Nodal proteins (0N), Mixer (0M) or Sox17 (0S), respectively. The number of
genes in each category is indicated in red. The simple linear model of
endoderm development predicts only coordinate positive regulation by Nodal
proteins, Mixer and Sox17 (A, blue box). The endoderm-enriched transcriptome
is complex and can be classified into 19 different categories. A, animal cap;
V, vegetal region; E, equatorial region; W, whole embryo.
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