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Fig. 7. Testing novel modes of endoderm gene regulation predicted by array
analysis. (A-H) At the two-cell stage, embryos were injected with
either Cerb-S RNA to inhibit nodal signaling (1 ng), antisense
morpholino oligos to Sox17
1+2+ß (Sox17-MO; 20 ng each) or
Mixer antisense morpholino oligos (40 ng/embryos). Some
Cerb-S-injected embryos were subsequently injected with
XtSox17ß RNA (10-100 pg), Mixer RNA (50-500 pg) or
Gata6 RNA (50-100 pg) to rescue target gene expression. A range of
rescue RNA doses was used and the lowest does that gave a reproducible rescue
is shown. At stage 11, RNA from the resulting embryos was assayed by real-time
RT-PCR for the expression of the indicated transcripts. Relative expression
normalized to ODC is shown and the expression level in control
gastrula was set to 1.0. Each experiment was repeated at least twice and a
representative experiment is shown. Potential regulatory pathways are shown
for each class of regulation (right).
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