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Figure 5


Fig. 5. TAGteam sequences affect SxlPe responsiveness. (A) Schematic of wild-type and TAGteam-mutant versions of the 1.4-kb SxlPe-lacZ reporter transgene. (B-E) Representative samples of ß-galactosidase antibody-stained 3- to 5-hour embryos (25°C) carrying the indicated transgene. (F-I) Distribution of ß-galactosidase antibody staining intensities among stage 8-11 embryos carrying the indicated transgene (n>2400 total embryos per construct, with four independent lines sampled for each). If all females and only females express SxlPe, 50% of the embryos should stain. Scale bar: 250 µm.





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