spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. TAGteam sequences affect the timing and penetrance of transcription from SxlPe. (A,C) In situ hybridization to lacZ RNA from a cycle 14 embryo carrying, respectively, the wild-type or the mutTAGteam 1.4-kb SxlPe-lacZ reporter transgene described in Fig. 5. (B,D) Nuclear dots of lacZ RNA hybridization in a cycle 13 embryo of the genotype as in A or C, respectively, co-stained for DNA (DAPI) to reveal nuclei. (E) Expression measured as the average number of dot-containing nuclei per presumed female embryo (n>=30 embryos per cycle per transgene). Arrows indicate onset of transcription (first cycle >=5% expressing nuclei). Dot analysis was performed on a single wild-type and TAGteam mutant transgene line, each determined to be representative based upon ß-galactosidase staining. Scale bars: A,C, 50 µm; B,D, 20 µm.





Right arrow Return to article