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Fig. 7. TAGteam sites in the zen ventral repression element (VRE) help
activate transcription. (A) Schematic of transgenes showing the
segments of the VRE that were used to drive an eve-lacZ reporter.
(B-G) lacZ RNA in situ hybridization to cycle 14 embryos
carrying the 600VRE (B), mut600VRE (C), 250VRE (D), 111VRE (E), mut111VRE (F),
or (2X)111VRE (G) transgene. (H) Nuclear RNA hybridization dots in a
cycle 7 embryo carrying the (2X)111VRE transgene, co-stained with DAPI for DNA
to reveal nuclei. The arrow indicates a dot-containing nucleus, which is
magnified in the inset. (I) Expression measured as the average
percentage of dot-containing nuclei per embryo. Transcription onset is defined
as the first cycle 
5%. Six lines were examined for the mut111VRE
construct, three for all others. For each nuclear cycle, 18 embryos were
assayed for the 250VRE construct, and 37 for all others. Scale bars: B-G,
50 µm; H-J, 20 µm.
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