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Figure 6


Fig. 6. Sens binds proneural proteins through its Zn-finger domains. (A) An IP assay shows that Sens interacts with Ac in COS-7 cells. (B) In vitro translated c-myc-tagged Sens interacts with bacterially expressed GST-tagged proneural proteins in a GST pull-down assay. Input lane shows 20% of the input used in the GST pull-down assay. (C) Sens binds to Sc through its Zn-finger domains. Bacterially expressed GST-tagged Sc protein can interact with in vitro translated full-length Sens (a), and Sens Zn-finger domains (c), but not with Sens N terminus (b), which lacks the Zn-finger domains. (D) Sens can still bind GST-tagged Sc if any of its Zn-finger domains are deleted (b,c,d,e). However, Sens loses its ability to bind Sc if Zn finger2, linker2 and Zn finger3 are deleted together (f). The bottom gel shows western blot analysis using anti c-myc antibody to detect 20% of the input. (E) Sens-L2-T-E binds Sc in a GST pull-down assay. The binding is weaker than wild-type Sens, in line with the weaker bristle phenotype (see Fig. 4B,C). Input lanes show 20% of the actual input.





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