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Figure 1


Fig. 1. Midline defects in the cerebellum and cerebellar primordium of Gbx2neo homozygotes. (A-C) Dorsal view of posterior E17.5 brains. The cerebellar midline (asterisks) in Gbx2neo homozygotes (B) is absent compared with control (A), whereas the entire cerebellum is absent in Gbx2{Delta}hb homozygotes (C). Insets in A,B show whole-mount ISH of Math1 expression at E11.5. In controls, Math1 is expressed in proliferative granule cell precursors in the cerebellar primordium (CEP; black arrow), including the vermis primordium (white arrowhead, A inset). Math1 expression is downregulated throughout the CEP and is absent along the vermis primordium of Gbx2neo homozygotes (inset in B). (D,E) Hematoxylin- and Eosin-stained coronal sections of E14.5 cerebella. The vermis primordium is greatly reduced in size and the vermal EGL is much thinner in Gbx2neo homozygotes (E) compared with controls (D). (F,G) Darkfield images of anti-Phospho-Histone H3 staining (white) on coronal sections of E14.5 cerebella. Mitotically active granule cell precursors are greatly reduced throughout the vermal EGL of Gbx2neo homozygotes (G). Insets in F,G show whole-mount ISH of Math1 expression at E13.5, illustrating the loss of midline granule cell precursors (arrowhead). Cb, cerebellum; Cp, choroid plexus; Mb, midbrain; Md, medulla; hEGL, hemispheric external germinal layer; ic, inferior colliculus; vEGL, vermal external germinal layer.





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