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Fig. 3. rdx is necessary for the meiotic to mitotic transition.
Cuticle from a wild type Canton-S embryo (A) shows evenly
spaced denticle bands on the ventral surface, telson structures at the
posterior end (t) and internal mouthparts at the anterior end (m). (B)
Hoechst staining of DNA visualizes mitoses in a wild type Canton-S
blastoderm stage embryo. The cuticle of a rdx6 GLC embryo
(C) shows intact telson (t), a fusion of A2-A8, intact T3 and A1
denticle bands, and a large anterior hole extruding malformed mouthparts (m).
In wild type (B), nuclei are uniformly spaced, synchronized divisions occur,
and chromatin is distributed evenly between daughter nuclei. In a
rdx6 GLC embryo (D), mitoses are asynchronous,
nuclear spacing is uneven, mitotic figures are highly abnormal (inset) and
much of the DNA has sunk into the interior of the embryo (internal staining is
out of focus).