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Fig. 2. Amphibian PouV proteins can support mouse ES cell self-renewal.
(A) The indicated PouV cDNAs were co-transfected with the Fgf4
enhancer or the octamer-binding motif luciferase reporters. Fold induction
represents the increase in transcription compared with the vector-only
control. Experiments were carried out in triplicate. (B) Morphology of
day 9 puromycin resistant colonies transfected with the indicated cDNA in the
presence or absence of Oct4 transgene expression. Colonies are stained for AP
activity (red). Scale bars: 100 µm. (C) Rescue index for PouV
proteins. The rescue index=the number of ES cell colonies in the absence of
Oct4 /number of ES cell colonies in the presence of Oct4. Mouse Oct4 was
assigned a value of 1 to which all other values were normalized. Data
represents the mean values obtained from three independent experiments.
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