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Figure 4


Fig. 4. Depletion of XlPouV proteins causes posterior truncation and anterior defects. (A) In vitro translation assay. The antisense MOs specifically prevent translation of their target mRNA but not the non-complementary (NC) mRNA. 3xMO indicates that a mix of all three XlpouV MOs was used. Capped mRNAs (1 µg) were used for translation and MOs were used at a concentration of 1 µM. (B) Two-cell stage embryos were injected in both blastomeres with control MO or XlpouV MO, and the octamer binding motif or the Fgf4 enhancer luciferase reporters. Embryos were assayed for luciferase activity at stage 9.5. The experiment was carried out in triplicate. (C) Two-cell stage embryos were injected in both blastomeres with 60 ng (control MO only) or 20 ng of each indicated MO and collected at stage 40. The term XlpouV MO was used to indicate that all three MO had been injected simultaneously.





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