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Fig. 7. Depletion of PouV proteins produces a heightened activin response in
animal cap explants. (A) Real-time RT-PCR analysis of XlpouV
protein-depleted animal cap explants. Embryos were injected with control MO or
XlpouV MO as above. Animal cap explants were isolated at stage 8 and cultured
with or without activin until sibling embryos reached stage 10.25. Relative
expression represents values normalized to Odc. 16 U/ml activin was
used for induction apart from the Xbra and Bmp4 analysis
that used 8 U/ml activin. Experiments were carried out in triplicate.
(B) Embryos were injected with control MO or XlpouV MO as above and
animal caps explants isolated and induced with either 8 U/ml or 16 U/ml
activin. Relative expression represents the Gsc and cerberus values
normalized to Odc. Experiments were carried out in triplicate.
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