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Figure 5


Fig. 5. A comparison of {alpha}8S50P/- {alpha}3-/- and {alpha}8-/- {alpha}3-/- lenses. (A) Toluidine Blue-stained E15.5 {alpha}8S50P/- {alpha}3-/- and {alpha}8-/- {alpha}3-/- embryonic lens sections show no space between the epithelium and posterior primary fiber cells (upper panels). Photos of P21 {alpha}8S50P/- {alpha}3-/- and {alpha}8-/- {alpha}3-/- lenses display large nuclear cataracts with transparent cortex (lower panels). (B) A Toluidine Blue-stained P14 {alpha}8S50P/- {alpha}3-/- lens section shows degenerating inner fiber cells and normal peripheral secondary fibers. Scale bars: 50 µm for E15.5 sections; 0.5 mm for P21 lenses; 100 µm for P14 sections.





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