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Fig. 5. Characteristics of the most primitive lymphoid progenitors and their
progeny in the fetal liver. (A) Fetal liver cells from E11.5
embryos were stained with APC-anti-CD45 (upper, left panel) and sorted into
CD45- and CD45+ fractions (upper, middle and right
panels). The sorted CD45+ cells were then stained with PE-anti-Kit,
PE-anti-Mac-1 or biotinylated anti-AA4.1 followed by PE-TR-streptavidin (lower
panels). Flow cytometry results are shown illustrating the expression of these
markers on RAG-1/GFP+ progenitors. (B) Progenitors from
E14.5 fetal liver were sorted on the basis of RAG1/GFP, Kit and Sca1
(Yokota et al., 2003) and then
cultured on OP9-control or OP9-DL1 for 14 days. Yields of CD19+ B
cells (dotted bars in the top panel), TCR
+ (white
bars, lower) or TCRß+ (hatched bars, lower) T cells per input
progenitors are shown. (C) All lymphoid progenitor containing subsets
of fetal liver at the indicated gestational stages were resolved. Percentages
of each cell type were multiplied by cell yields to obtain absolute numbers
per organ.
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