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Figure 3


Fig. 3. Basonuclin-RNAi transgene affected the morphology of ovary and oocytes. Histology of non-transgenic (A,B) and T50 transgenic (C,D) ovaries are shown at two magnifications: the entire ovary (A,C) and an enlarged region (B,D). Arrows indicate follicles that contain abnormal oocytes; arrowheads indicate remnants of degenerating follicles. Follicles and oocytes are apparently normal in the transgenic ovary (*). cl, corpus luteum. GV-stage oocytes were isolated and observed under the phase-contrast (E,F) and differential interference contrast (DIC) (G,H) microscopy. Non-transgenic oocytes are shown in E,G, and transgenic ones in F,H. Arrowheads indicate examples of dark granules in the transgenic cytoplasm (F) and the bumps on transgenic cell surface (H). The transgenic population is heterogenous: both morphologically normal and abnormal oocytes are present. Scale bars: 50 µm in E; 10 µm in H.





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