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Figure 6


Fig. 6. Pre-implantation development of basonuclin-RNAi transgenic embryos. Eggs fertilized in vivo were collected and cultured in vitro for 84 hours (3.5 days). (A) Development of the transgenic (T50) embryos was arrested at the two-cell stage. (B) At 36, 60 and 84 hours post-coitum, the developmental stages of the embryos were scored and expressed as a percentage (±s.d.) of the population. The data are the average of three experiments, in which a total of 40-60 embryos for each transgenic line were scored. The dead category includes collapsed, degenerated or fragmented embryos; 1C, one cell, 2C, two cell (etc.); M/B, morula/blastocyst.





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