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Figure 1


Fig. 1. Overexpression of SOX10 inhibits overt neuronal and glial differentiation of EPCs. (A,B) Staining of wild-type EPC colonies expressing either GFP (A) or SOX10-GFP (B) transgenes with antibodies for GFP (green) and TuJ1 (red). (C) Percentage of TuJ1+ cells present in GFP- (green bars) or SOX10-GFP-(blue bars) expressing EPC colonies 5, 7 and 10 days after plating. (D-K) Day 5 EPC colonies expressing GFP (D,F,H,J) or SOX10-GFP (E,G,I,K) transgenes were immunostained for GFP (D,E,H,I), MASH1 (F,G) or RET (J,K). Optic fields in D,E and H,I are the same as those shown in F,G and J,K, respectively. (L,M) Day 10 GFP or SOX10-GFP colonies double immunostained for GFP (green) and GFAP (red).





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