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Fig. 2. EDN3 signalling inhibits lineage commitment and differentiation of
EPCs. Wild-type EPCs were isolated from cultures of embryonic
(A-D,H-K,L-O,R,S) or postnatal (E,F,P,Q,T,U) gut and maintained in either
standard (control) medium (CM; A,C,E,H,J,L,N,P,R,T) or CM supplemented with 10
nM EDN3 (B,D,F,I,K,M,O,Q,S,U). Colonies were fixed 5 (A,B,H-K,L,M) or 10
(C-F,N-Q,R-U) days after plating and immunostained for TuJ1 (A-F),
MASH1 (H,I), RET (J,K), B-FABP
(L,M), GFAP (N-Q) and SOX10 (R-U). In all panels,
colonies were counterstained with DAPI. (G) Percentage of
TuJ1+ cells in colonies maintained either in standard medium (CM;
yellow bars) or medium supplemented with 10 nM EDN3 (CM+EDN3; green bars) 5, 7
and 10 days after plating. (V) Percentage of SOX10+ cells in
colonies maintained in CM (yellow bars) or CM+EDN3 (green bars) 1, 5 or 10
days after plating.
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