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Figure 5


Fig. 5. Reduction of the pool of ENS progenitors in Edn3ls/ls embryos. (A) Whole-mount preparation of gut from Edn3+/+; TgWnt1Cre; R26YFPStop embryos immunostained for YFP. Box indicates the part of gut used to generate the intestinal acute cultures. (B) Bars represent the fraction of double positive (SOX10+YFP+) cells relative to the total population of neural crest-derived (YFP+) cells in acute intestinal cultures established from embryos wild-type (red) or heterozygous (blue) and homozygous for Edn3ls (green).





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