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Fig. 6. Reduced EDN3 signalling decreases the proliferation of specific
subpopulations of enteric neural crest cells. (A) Whole-mount
preparation of gut from Edn3+/+;
TgWnt1Cre; R26YFPStop embryos
immunostained for YFP. Box 1 indicates the part of gut used to generate the
intestinal acute cultures examined in C and D. Box 2 indicates the area of the
gut analysed in B and E. (B) Confocal image of an embryonic gut region
corresponding to box 2 of A. Gut was immunostained for BrdU (red) and YFP
(green). (C) Bars represent the fraction of
BrdU+YFP+ cells relative to the total population of
neural crest-derived (YFP+) cells in acute intestinal cultures from
wild-type (red), Edn3+/ls (blue) and
Edn3ls/ls (green) embryos. (D) Percentage of
BrdU+SOX10+ cells within the total population of
SOX10+ cells in acute intestinal cultures from wild-type,
Edn3+/ls and Edn3ls/ls embryos.
(E) Fraction of BrdU+YFP+ cells relative to the
total population of YFP+ cells at the migratory wavefront of
enteric neural crest cells.
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