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Figure 7


Fig. 7. Increased neuronal differentiation of enteric neural crest cells at the migratory front. (A,B) Whole-mount preparations of guts from E11.5 TgWnt1Cre;R26YFPStop embryos wild-type (Edn3+/+; A) or homozygous for the ls allele (Edn3ls/ls; B); guts were immunostained for YFP (green) and TuJ1 (red). Images correspond to the front of enteric neural crest cell migration (part of box 2 of the gut shown in Fig. 6A). (C) Bars represent the fraction of double-positive (TuJ1+YFP+) cells within the total population of YFP+ cells present at the front of migration of wild-type (red), Edn3+/ls (blue) and Edn3ls/ls (green) embryos.





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