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Fig. 3. dILA neurons are the product of asymmetric cell divisions.
(A) Summary of all possible cell divisions that could generate dILA or
dILB neurons. Yes/no indicates if a particular type of progenitor cell
division was observed by lineage tracing experiments using retroviral
infection. (B) Quantification of the different types of independent
two-cell clones observed after retroviral infection. (C-G) Two-cell
clones residing in the dorsal spinal cord were identified after retroviral
infection by the expression of ß-galactosidase, as assessed by
immunohistochemisty (green). The molecular identity of cells was assessed by
the use of antibodies directed against Lbx1 (red) and Lhx1/5 (blue). dILA
neurons were defined as Lbx1+/Lhx1/5+ cells, dILB neurons as
Lbx1+/Lhx1/5– cells, and progenitors as Lbx1-negative cells located
close to the ventricle. Only those clones that contained at least one Lbx1+
neuron were assessed. Spinal cords are shown at low (C) and high (D-G)
magnification. (E) High magnification of a two-cell clone containing one dILA
(white) and one dILB (yellow) neuron. (G) High magnification of a two-cell
clone containing two dILB neurons (yellow). Scale bar: 100 µm in A;10 µm
in B-E.
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