QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. Effect of the Mash1 mutation on the generation of dILA neurons. Dorsal neurons in control, Mash1^–/– and Mash1^Ngn2/Mash1^Ngn2 embryos were analyzed at E12.5 using immunohistochemistry, with antibodies directed against (A-F) Lhx1/5 (green) and Tlx3 (red), (H-J) Pax2 (green) and Lmx1b (red). dILA neurons express Lhx1/5 and Pax2, and dILB neurons express Tlx3 and Lmx1b. (G,K) Numbers of BrdU+ dILA (dark-grey columns) and BrdU+ dILB (light grey columns) neurons in control (c), Mash1^–/– (^–/–) and Mash1^Ngn2/Mash1^Ngn2 (N2/N2) mutant mice, as assessed 24 hours after BrdU injection. The number of dILA neurons is reduced in Mash1^–/– embryos. (L-N) Analysis of Ptf1a (green) and Mash1 (red) expression in the dorsal spinal cord of control (L), Mash1^–/– (M) and Mash1^Ngn2/Mash1^Ngn2 (N) embryos. (O) Numbers of Ptf1a+ cells (black columns) in control (c), Mash1^–/– (^–/–) and Mash1^Ngn2/Mash1^Ngn2 (N2/N2) mutant mice. The error bars represent the standard deviation. (P-R) In situ hybridization of sections from E12.5 control, Mash1^–/– and Mash1^Ngn2/Mash1^Ngn2 spinal cords using a probe directed against Hes5 mRNA. Scale bars: 100 µm in A-C,P-R; 50 µm in D-F,H-J,L-N.

Return to article