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Fig. 2. ISNb motor axon pathfinding defects in plexB mutants resemble plexA mutants. (A-F) Filleted preparations of late stage 16 embryos stained with the anti-Fasciclin II monoclonal antibody to reveal motor axons of the ISNb. Arrows and open arrows indicate proper and absent innervation, respectively, by axons of the ISNb. Anterior, left; dorsal, up. (A) In a wild-type embryo, axons within the ISNb innervate the ventral longitudinal muscles 12, 13, 6 and 7 (arrows). (B) ISNb motor axons fail to defasciculate in plexA^Df(4)C3 mutants and often do not innervate their proper muscle targets (open arrows). (C) plexB^KG00878 mutant ISNb motor axon pathways, like plexA^Df(4)C3 mutants, also fail to reach their proper muscle targets (open arrows). (D) Neuronal expression of plexB in a plexB^KG00878 mutant background restores proper neuromuscular connectivity (arrows). (E) Neuronal plexB expression in a plexA^Df(4)C3 mutant background completely fails to rescue the plexA^Df(4)C3 mutant phenotype (open arrows). (F) Neuronal plexA expression in a plexB^KG00878 mutant background partially rescues the plexB^KG00878 mutant phenotype (absent, left, and normal, right, innervation are shown). (G) Schematics of two adjacent hemisegments illustrating ISNb phenotypes observed in wild type (left), plexA mutants (middle) and plexB mutants (right). Scale bar in A: 10 µm for A-F.

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