spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. GATA2 expression is turned on in newborn progenitors and is sufficient to arrest proliferation. (A) Transverse section of the caudal hindbrain of a E10.5 mouse embryo, first hybridised with Gata2 anti-sense RNA (green), then stained with antibodies against Isl1 (red), a pan-marker of motoneurons. The presumptive domain of V2 precursors, which express Gata2, is located between the respective domains of the motoneuron and V1 interneuron precursors; in the hindbrain, Gata2 is also activated to a lesser extent in the p3 domain, indicated by the white arrow. (B,C) Transverse section of the spinal cord of a E10.5 mouse embryo injected with BrdU. Hybridisation with Gata2 antisense RNA (B) was followed by BrdU immunostaining (C). (D-F) Higher magnification of the area included in the white rectangle in C. (G-L) Transverse sections of the spinal cord of chick embryos 24 hours after electroporation with the pAdRSV-GATA2HA plasmid, double-stained with anti-HA antibodies (G,J) and either with anti-phospho-Histone3 (pH3) (H) or anti-BrdU antibodies (K), and analysed by confocal microscopy. (I,L) Superimpositions of G,H (I) and J,K (L). (M,N) The percentages of phospho-Histone3- and BrdU-positive cells in the control (red, M) and the transfected sides (blue, N) are compared. (O,P) Adjacent sections of chick embryos misexpressing GATA2-HA, stained with anti-HA-antibodies and hybridised with chick Sox2 anti-sense RNA. Scale bar: in A, 70 µm in A-C; in D, 20 µm in D-F; in G, 20 µm in G-L; in O, 50 µm in O,P.





Right arrow Return to article