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Figure 9


Fig. 9. BMP signaling enhances expression of the pronephric marker pax2.1 in lateral mesoderm. (A-E) Representative embryos derived from fertilized eggs that were control (A,D) or had been injected with the I(lmo2:{Delta}BR)I transgene to express the dominant negative receptor (B,E) or the I(lmo2:caBR)I to express the constitutively active receptor (C). Embryos shown in A-C were analyzed for expression of the pronephric marker pax2.1 (arrowheads) and co-stained for the expression of the midline mesoderm marker ntl as an internal control (asterisks). Embryos in D and E were processed sequentially to detect the expression of pax2.1 (purple) and gata1 (red). The magnified views in the side panels of D and E demonstrate that the gata1 pattern is expanded (white bars) at the expense of the pax2.1 pattern (black bars) in injected embryos. Views are flat-mounted dorsal, anterior towards the top.





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