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Figure 1


Fig. 1. DMPP is lethal at the L2/L3 molt and induces aberrant L3 cuticle synthesis. (A) Fraction of wild-type larvae reaching adulthood when grown on increasing concentrations of DMPP (n≥50 worms). Experiments were performed at 20°C. (B) Representative example of an animal exposed to 0.75 mM DMPP which dies as an early L3 larvae, just after shedding the L2 cuticle (arrow). Scale bar: 0.1 mm. (C-E) Electron microscopy (EM) pictures after chemical fixation of young L3 larvae. Scale bar: 5 µm. Worms grown on DMPP (D,E) have irregular shapes when compared with the control (C). (F,G) Electron microscopy pictures of young L3 larvae cuticle after high-pressure freezing fixation. Scale bar: 250 nm. (F) Control animals grown on standard plates show a typical mono-layered L3 cuticle (Cut) covering a thin epidermis (Ep) and underlying body-wall muscles (Mus). (G) Animals grown on DMPP have an aberrant L3 cuticle with several layers of heterogeneous aspects and fibrillar structures (arrow).





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