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Fig. 3. Forcing development through an L2 arrest suppresses DMPP-induced
lethality. (A) Worms grown on a low amount (80 µg) of
heat-killed bacteria arrest development as L2 larvae. Bars represent the % of
larvae at each developmental stage, mean of two independent experiments.
(B,C) DIC pictures of a mid-L2 larva grown on live E.
coli (B) and an arrested L2 larva grown on 80 µg heat-killed E.
coli (C). Arrested L2 larvae show a seam cell pattern typical of a worm
having completed the two successive L2 divisions without extensive anterior
sister cell migration. There is high storage droplet content in the epidermis
(C). Scale bar: 10 µm. (D) Schematic representation of worm
development on low amount of heat-killed E. coli as schematized in
Fig. 2D. (E) DMPP
resistance of animals after L2 arrest. Both groups were grown on 0.75 mM DMPP.
Worms transferred on standard plates after a 3-day arrest in L2 were partially
resistant to DMPP (`3 days L2 diapause') when compared with control
(`continuous development'). Error bar represents s.e.m., n=3
independent experiments.
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