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Figure 4


Fig. 4. UNC-63-containing nAChRs are required to implement the DMPP signal. (A) Survival on 0.75 mM DMPP. Error bars represent s.e.m. (n≥3). Punc-63::unc-63: extrachromosomal array carrying an unc-63 genomic fragment; Pmyo-3::unc-63: muscle specific promoter driving the expression of an unc-63 cDNA. unc-63(kr13);Punc-63::unc-63, unc-38(x38) and unc-29(e1072) are significantly less DMPP resistant than unc-63(kr13) (*: P< 0.05, Mann-Whitney test). Pmyo-3::unc-63 does not rescue unc-63(kr13) DMPP resistance while it restores wild-type locomotion and levamisole sensitivity (see Fig. S1 in the supplementary material). (B-D) unc-63 expression profile. Confocal picture of a transgenic larva expressing Punc-63::unc-63-SL2-GFP. unc-63 is expressed in body-wall muscles (B, arrow), head muscles (C, arrowhead), and in many neurons in the head (C) and the ventral cord (D, arrow). Scale bar: 20 µm. (E) unc-63(kr13) does not alter the timing of L1/L2 and L2/L3 molts. Each dot represents the percentage of worms pumping at a given time (n>25 individuals). Broken lines indicate the timing of wild-type events.





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