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Figure 5


Fig. 5. DMPP resistance of daf-12 and daf-9 mutants. (A) daf-12 gene structure. Mutation sites of new Mos1 alleles and alleles tested for DMPP resistance are indicated. *, STOP codon. (B) Dose-response sensitivity to DMPP of wild-type and daf-12(rh61rh411) mutants. (C) daf-12(rh61rh411) does not alter the timing of L1/L2 and L2/L3 molts. Each dot represents the percentage of worms pumping at a given time (n>25 individuals). broken lines indicate the timing of wild-type events. (D) daf-12(rh61rh411) is insensitive to DMPP-induced developmental delay. L2 development was divided into five stages based on seam cell (SC) divisions and anchor cell (AC) differentiation (see Fig. 2B). Developmental stage was monitored using DIC optics, which did not allow the discrimination between classes 3 and 4. The proportion of worms belonging to each class was scored 32 hours after egg laying (n≥12). Data presented are from one representative experiment out of three independent trials. (E) DMPP resistance of daf-12 and daf-9 mutants (0.75 mM DMPP). Error bars represent s.e.m. (n≥3). Table presenting dauer and heterochronic phenotypes [adapted, with permission, from Antebi et al. (Antebi et al., 1998)]. dtc, distal tip cell.





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