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Figure 1


Fig. 1. Crz expression in the CNS during metamorphosis. (A,B) In situ hybridization of Crz mRNA. (A) Third-instar larval CNS, showing three pairs of DL neuron in the brain (Br) and eight pairs of vCrz neurons (brackets). (B) Pupal CNS at ~36 hours APF. The number of neurons in the brain (arrowhead) is found to be six to eight per lobe, whereas none of vCrz neurons are detectable. (C,D) Crz-IR neurons in third-instar larval CNS (C) and pupal CNS at 12-24 hours APF (D). Consistent with the in situ hybridization results, no Crz-IR neurons are detectable in the VNC of the pupal CNS (arrow). At least five specimens were processed for each panel. (E) Colocalization (center) of Crz-gal4-induced GFP expression (left) in Crz-IR neurons (right). Scale bars: 100 µm.





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