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Fig. 4. Inhibition of vCrz-cell death by targeted expression of EcR-B1 dominant-negative forms. (A-C) CNSs were dissected at 12 hours APF and processed for X-gal staining. (A) Control (UAS-lacZ/+; Crz-gal4/+). An arrowhead indicates the staining from residual ß-galactosidase activity. (B) UAS-lacZ/+; Crz-gal4/UAS-EcR-B1^F645A (n=4). (C) UAS-lacZ/+; Crz-gal4/UAS-EcR-B1^W650A (n=4). Live vCrz neurons are shown within the brackets. (D) (Left) A confocal image showing nuclear EcR-B1-IRy (green) in a vCrz neuron (orange, arrow) at white prepupal stage of wild-type. (Right) An independent specimen showing two vCrz neurons containing EcR-B1-IRy (arrows). (E-H) Crz-IRy in various EcR mutants at 12-17 hours APF. (E) Wild type. (F) EcR-A mutant (EcR^M554fs/EcR¹¹²). (G) EcR-B mutant (EcR³¹/EcR⁹⁹) after completion of apolysis. Surviving vCrz neurons are shown in the brackets (see also Fig. 5A). (H) EcR-B1 mutant (EcR³¹/EcR^Q50st). Scale bars: in A, 50 µm for A-C,E-H; in D, 10 µm for D.

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