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Fig. 6. The role of rpr in vCr2 PCD. (A-D) Crz-IRy in (A) H99/TM6B, (B) XR38/TM6B, (C) rpr-null mutant (XR38/H99) at 1216 hours APF, and (D) hid loss-of-function mutant (X14/hid⁰⁵⁰¹⁴) at 8 hours APF. Note that 14 vCrz neurons are still detectable in the rpr mutant (brackets in C). (E,F) Rpr in situ hybridization of the CNSs dissected from (E) third-instar larva (Crz-gal4, UAS-p35/UAS-rpr) and (F) wild-type prepupa at 1.5 hours APF. The hybridization signals are indicated by arrowheads or by a circle. (G) Double labeling of wild-type CNS with rpr in situ hybridization probe (top) and anti-Crz (middle) at 1.5 hours APF. Merge of the two images (bottom) shows co-localization of rpr mRNA in a vCrz neuron (arrow). Expression of rpr in non-vCrz neurons is indicated by arrowheads. (H) rpr promoter activity in vCrz neurons at 1 hour APF. Crz-IRy (top), rpr-gal4-driven GFP expression (middle) and merge of the two (bottom). A vCrz neuron positive for both GFP and Crz-IRy appears in yellow. (I) Rpr-gal4-mediated p35 expression (rpr-gal4/UAS-p35) suppresses the death of 10 vCrz neurons (brackets) at 7-9 hours APF. Two different specimens are shown here. Scale bars: in D, 50 µm for A-D,I; in E, 50 µm for E,F; 10 µm in G,H.

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