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Fig. 4. Robo1 knockout mice display aberrant axonal pathfinding
in the corpus callosum and hippocampal commissure. (A-H) Coronal
brain sections taken at the rostral level of littermates of Robo1
mice were labelled with L1-CAM immunohistochemistry to reveal axonal
projections in the corpus callosum (CC) and fornix/hippocampal commissure at
E17.5. In wild-type (A,B,E,F) and heterozygous (C,D,G,H) mice, both callosal
and hippocampal projections appeared normal. However, in knockout littermates,
medially projecting axons formed tight fascicles that failed to cross the
midline (white arrows in K,K',L,L'). Sections of the same brain
from rostral (I,K) to caudal (J,L) indicated that
axons along the rostrocaudal extent of these commissures were disrupted (I-L).
Some axons still crossed the midline (asterisks in L,L'), indicating
that not all callosal axons were affected. E-H and K,L are higher power images
of A-D and I,J, respectively. K' and L' are higher power views of
the boxed regions in K and L, respectively. Scale bars: in J, 200 µm for
A-D,I,J; in L, 100 µm for E-H,K,L and 50 µm for K',L'.
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