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Fig. 4. Time course of GrlE expression. (A) Total RNA was extracted
from wild-type AX4 cells harvested at the indicated time of development and
electrophoretically separated on a 1.2% agarose gel before being transferred
to a nylon membrane. The membrane was hybridized with a probe for
grlE (see Materials and methods). (B) Samples were collected
from wild-type and grlE– null cells that had
developed for 0, 4 and 16 hours, and probed for grlE mRNA. Consistent
loading in each lane was confirmed by staining for rRNA.
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