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Figure 3


Fig. 3. Pbx1 and Pbx2 colocalize in lateral plate-intermediate mesoderm and early limb fields. (A,E,I) At E8.5, Pbx1 (A) and Pbx2 (E) colocalize (I) in lateral plate-intermediate mesoderm (red arrowheads). (B,F,J) At E9, Pbx1 (B) and Pbx2 (F) colocalize (J) in the early limb fields (red arrowheads). (C,G,K) At E9.25, although overlapping proximally (K), Pbx1 (C) and Pbx2 (G) forelimb expression domains become mostly complementary, being proximal and distal, respectively (red arrowheads). (D,H,L) At E9.25, Pbx1 (D) and Pbx2 (H) hindlimb expression domains remain overlapping (L) (red arrowheads). Proximal is leftwards; anterior is towards the top. som, somites.





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