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Fig. 1. Reduction of Bmp signaling at the mid-gastrula stage causes expansion of
blood and vascular cells into the extreme ventral mesoderm. (A-I)
gata1 (A-C), flk1 (D-F), and pax2.1 (G-I)
expression in heat-shocked wild-type and transgenic sibling embryos.
Transgenic embryos heat shocked at dome stage have a loss or severe reduction
in the expression of all three markers (B,E,H). Transgenic embryos heat
shocked at 80% epiboly (mid-gastrula) have an expansion of gata1 and
flk1 expression into the mesoderm below the tail bud (tb), marked by
an arrow. All embryos are flat-mounted with anterior to the top, and images in
A,D,G are representative of wild-type embryos from both stages of heat
shock.
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