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Fig. 2. Lineage labeling of cells ventral to the tail bud reveals severe
deficiencies in ventral tissues of transgenic embryos. (A) Scheme
for the lineage labeling of extreme ventral cells. (B) Illustration of
the labeled region depicted in C and D. Note the decreased
number of red fluorescent cells in the ventral yolk extension (bracketed) and
cloacal (boxed) region in D compared with in C. (E,F)
Visualization of 24 hpf embryos with Nomarski optics revealed severe defects
in cloaca formation in transgenic embryos (F) compared with wild-type siblings
(E). Arrowhead in (E) depicts the cloacal aperture, and boxed regions in (E)
and (F) highlight the pronephric terminus.
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