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Fig. 3. Reduced ventral gene expression in transgenic embryos.
(A-C,E-G) 10-somite wild-type (A-C) and transgenic (E-G) embryos
stained for hrT gene expression. A,B,E,F are lateral views; C,G are
flat mounts. `hf' in A,E labels the heart-field expression of hrT,
which is normal in transgenic embryos compared with wild-type siblings. The
boxed region highlights the extreme ventral mesoderm, enlarged in B and F;
arrowheads denote the ventral mesodermal domain. The bracketed regions in C
and G show this same region in flat-mounted embryos. (D,H)
18-somite-stage wild-type (D) and transgenic (H) embryos stained for
hrT expression. Arrowheads indicate the ventral mesodermal
hrT expression. (I,M) 18-somite-stage embryos stained
for tbx2b expression in wild-type (I) and transgenic (M) embryos.
Brackets indicate extreme ventral tbx2b expression in the proctodeal
and ventral yolk regions; this expression is absent in the transgenic embryos.
(J,N) 18-somite stage embryos stained for hoxd13 in
wild-type (J) and transgenic (N) embryos. Arrowheads indicate the proctodeal
region, where hoxd13 expression is absent in transgenics compared
with wild-type siblings. Note that the posterior expression of hoxd13
expands anteriorly in the transgenic embryos. (K,O)
gata3 expression in wild-type (K) and transgenic (O) embryos.
Arrowheads indicate the ventral limit of the normal pronephric terminus.
(L,P) vox expression in wild-type (L) and transgenic
(P) embryos. Note the loss of ventral expression in transgenic embryos
compared with wild-type siblings. (Q-T) prdm1 (Q,S) and
evx1 (R,T) expression in wild-type and transgenic embryos at 24 hpf.
Note the loss of prdm1 expression in the epidermis and cloaca of
transgenic embryos. evx1 expression is absent in the cloaca of
transgenic embryos, but it is retained in the posterior gut and posterior
kidney tissues. Arrows in Q and R mark the cloacal domain of prdm1
and evx1 expression, respectively, and arrowheads in R and T mark the
posterior gut and kidney expression of evx1.
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