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Figure 6


Fig. 6. The number of muscle satellite cells is reduced in the heat shocked pax7EnR transgenic tails. (A) RT-PCR detection of pax7, pax6 and pax7EnR in wild-type and pax7EnR transgenic tadpoles with or without heat shock treatment. (B-E) The expression of Pax7 (red) in transverse sections of the tails. (B) Wild-type tail. (C) Wild-type regenerating tail with daily heat shock for 7 days after amputation. (D) pax7EnR transgenic tail. (E) pax7EnR transgenic regenerating tail with daily heat shock for seven days after amputation. (F) The histogram shows the number of satellite cells quantified by Pax7 antibody staining. Ten tails of similar size were examined in each case. **P<0.05. The d7 bars refer to 7-day regenerates. The d14 bars refer to 14-day regenerates amputated at a more distal level to provoke a second regeneration. Because of the more distal position, the absolute cell numbers per section are lower, but the wild type still has about twice the number of satellite cells compared with the heat-shocked pax7EnR. Scale bars: 20 µm.





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