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Fig. 2. Expression of full-length and deleted ft and ds constructs. (A-F) Expression of full-length and deleted ft and ds constructs in the posterior compartments (right) of ft^G-rv/ft^fd (A-C), ds⁰⁵¹⁴² (D,E) or wild type (F) wing imaginal discs with en-gal4. (A) UAS-ft. (B) UAS-ft ${Delta}$ ICD. In both A and B, Ft or the HA-tagged Ft ${Delta}$ ICD (green and center panels) is concentrated at the cell cortex, and leads to stronger anti-Ds staining (purple and right panels) in the region of misexpression. (C) UAS-ft ${Delta}$ ECD; anti-Ft staining (green and center panel) is diffuse and not concentrated at the cell cortex, and does not lead to stronger anti-Ds staining (purple and right panel) in the region of misexpression. There appears to be a slight decrease in Ds levels. (D) UAS-ds. (E) UAS-ds ${Delta}$ ICD. In both D and E, anti-Ds staining (green and center panels) is concentrated at the cell cortex, and leads to stronger anti-Ft staining (purple and right panel) in the region of misexpression. (F) UAS-ds ${Delta}$ ECD; anti-Ds staining (white) is diffuse and not concentrated at the cell cortex. (G) AyGal4 FLPout clone expressing UAS-ds ${Delta}$ ECD and UAS-GFP (green) in a wild-type wing imaginal disc. The clone does not lead to stronger anti-Ft staining (purple and right panel).

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